Journal: International journal of molecular sciences

Article Title: Microplastics Exacerbate Cadmium-Induced Kidney Injury by Enhancing Oxidative Stress, Autophagy, Apoptosis, and Fibrosis.

doi: 10.3390/ijms232214411

Figure Lengend Snippet: Figure 2. Effect of MPs and Cd on oxidative stress in mouse kidneys. Mice were treated with Cd (50 mg/L) and/or 5 µm MPs (10 mg/L) for 90 days. (A–D): The levels of catalase (CAT) activity, glutathione (GSH), and malondialdehyde (MDA were evaluated in the mouse serum. (E–H): the levels of sirtuin 3 (Sirt3), heme oxygenase 1 (HO-1), and superoxide dismutase 2 (SOD2) were detected using western blotting. (E): Representative western blot image; (F–H): Quantitative analysis. Data are presented as means ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to the control group; # p < 0.05 compared to the Cd group.

Article Snippet: Anti-sirtuin 3 (Sirt3) (sc-365175), anti-heme oxygenase 1 (HO-1) (sc-136960), and anti-superoxide dismutase 2 (SOD2) (sc-137254) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activity Assay, Western Blot, Control

Journal: Antioxidants

Article Title: Phosphatidylserine Counteracts the High Stocking Density-Induced Stress Response, Redox Imbalance and Immunosuppression in Fish Megalobrama ambylsephala

doi: 10.3390/antiox13060644

Figure Lengend Snippet: Hepatic protein expressions of sirtuin 3 (Sirt3, A ), superoxide dismutase 2 (Sod2, B ), and nuclear factor erythroid-2-related factor 2 (Nrf2, C ) in blunt snout bream subjected to different stocking densities and phosphatidylserine supplementation. SD, stocking density; PS, phosphatidylserine; ND, normal density; NDPS, ND supplemented with 50 mg/kg PS; HD, high density; HDPS, HD supplemented with 50 mg/kg PS. Each datum represents the mean of three replicates. The two-way ANOVA result is indicated by asterisks. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant.

Article Snippet: Anti-beta-actin (42 KDa, 1:1000, 66009-1-Ig, Proteintech, Wuhan, China), anti-sirtuin 3 (Sirt3) (28 KDa, 1:2000, Proteintech, 10099-1-AP, Wuhan, China), anti-superoxide dismutase 2 (Sod2) (25 KDa, 1:1000, Proteintech, 24127-1-AP, Wuhan, China), and anti-nuclear factor erythroid-2-related factor 2 (Nrf2) (68 KDa, 1:2000, Proteintech, 16396-1-AP, Wuhan, China) were all used.

Techniques:

Journal: Medicine and science in sports and exercise

Article Title: Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver

doi: 10.1249/MSS.0000000000001887

Figure Lengend Snippet: Mitochondrial protein acetylation mediates mitochondrial efficiency following DOX treatment in isolated liver mitochondria. a) Mitochondrial protein acetylation in isolated liver mitochondria from Sedentary Control, Sedentary DOX, Exercise Control and Exercise DOX treated animals. b) Relationship between mitochondrial efficiency (respiratory control ratio) and mitochondrial protein acetylation from Sedentary Control (black circle), Sedentary DOX (gray circles), Exercise Control (black triangles) and Exercise DOX (gray triangles) animals. c) Sirtuin 3 (Sirt3) protein content in isolated liver mitochondria from Sedentary Control, Sedentary DOX, Exercise Control and Exercise DOX treated animals. Values are mean ± SEM. *, P < 0.05 vs. all groups. d) Relationship between mitochondrial efficiency (respiratory control ratio) and mitochondrial Sirt3 protein content from Sedentary Control (black circle), Sedentary DOX (gray circles), Exercise Control (black triangles) and Exercise DOX (gray triangles) animals. e) Representative immunoblot images for acetylated lysine, Sirt3, and loading control VDAC.

Article Snippet: Membranes were incubated with the following antibodies: acetylated lysine (#9441) (Cell Signaling, Danvers, MA), citrate synthase (ab96600) (Abcam, Cambridge, MA), mitochondrial heat shock protein 70 (ab2799) (mtHSP70) (Abcam), mitochondrial transcription factor A (TFAM) (DR1071) (Calbiochem, Darmstadt, Germany), nuclear respiratory factor 1 (Nrf1) (200-401-869) (Rockland Immunochemicals, Limerick, PA), p62 (ab56416) (Abcam), Parkin (ab77924) (Abcam), PTEN-induced putative kinase 1 (PINK1) (ab23707) (Abcam), sirtuin 3 (Sirt3) (sc-365175) (Santa Cruz Biotechnology, Dallas, TX) and total OXPHOS (ab110413) (Abcam).

Techniques: Isolation, Western Blot